Degradation of newly synthesized collagen.
نویسندگان
چکیده
منابع مشابه
Degradation of newly synthesized collagen.
Explants of rabbit lung parenchyma maintained in serumfree medium incorporate I 14Clproline and I Wllysine into collagen as I “Clhydroxyproline and I Wlhydroxylysine at a constant rate for at least 24 h. Evaluation of the size distribution of the [14Clhydroxyproline and [‘4Clhydroxylysine containing peptides within the explants demonstrated that 20 to 40% of the LY chains of newly synthesized c...
متن کاملRegulation of the production of secretory proteins: intracellular degradation of newly synthesized "defective" collagen.
Confluent cultures of human fetal lung fibroblasts degrade approximately 10% of their newly synthesized collagen within the cell prior to secretion. This basal level of intracellular degradation could not be inhibited by colchicine or cytochalasin B, inhibitors of microtubular and microfilament function, respectively, or by N(alpha)-p-tosyl-L-lysine chloromethyl ketone, chloroquine, or NH(4)Cl,...
متن کاملAge-related changes in collagen synthesis and degradation in rat tissues. Importance of degradation of newly synthesized collagen in regulating collagen production.
During developmental growth, collagens are believed to be continuously deposited into an extracellular matrix which is increasingly stabilized by the formation of covalent cross-links throughout life. However, the age-related changes in rates of synthetic and degradative processes are less well understood. In the present study we measured rates of collagen synthesis in vivo using a flooding dos...
متن کاملNewly Synthesized Vasorelaxant
Effects of ER-001533 (ER), a newly synthesized vasorelaxant, on the membrane currents were examined in single ventricular cells of guinea pigs. The patch-clamp technique was used in the "whole-cell" and "inside-out" patch configurations. In the whole-cell clamp condition, ER induced a time-independent K'-dominant current, which was inhibited by glibenclamide (1-3 ,uM), suggesting that ER activa...
متن کاملIn vitro synthesis of collagen prolyl hydroxylase. The newly synthesized enzyme contains two newly synthesized subunits of the alpha and beta size.
Neonatal rat lung prolyl hydroxylase was synthesized in a cell-free system. Total polysomes were isolated from neonatal rat lung homogenates after Triton X-100 and deoxycholate solubilization. The polysomes were then translated in a wheat germ lysate in the presence of tritiated leucine. The radioactive product was precipitated with antibody to rat prolyl hydroxylase and co-precipitated with pr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1978
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)34727-0